Gluconacetobacter johannae

Genus/species (aliases): Gluconacetobacter johannae


Gram Stain: Negative


  • Cell: rods with rounded ends, approximately 1.5-1.9 micrometer and 0.5-0.6 micrometer; occur singly, in pairs or in short chains. Motile by means of peritrichous flagella.
  • Colony: light brown to brown colonies.
  • Liquid Growth:

Physiological Traits:

Growth on:  good growth on ethanol, galactose, xylose,; moderate growth on raffinose, maltose; faint growth on arabinose, butanol, glycerol, mannitol, melibiose, sorbitol;  no growth: mannose

Ecological Traits:

Found in nature associated with plant materials; tolerant of  low pH and high concentrations of sugar found on grapes.

Distinguishing Features:


Role in wine: 

  Found in musts.


  • SO2:
  • Sorbate­­­­­:
  • DMDC:
  • pH: Yes
  • Acids: Yes
  • Ethanol: Yes
  • Anaerobiosis:
  • Heat: Yes


Bergey’s manual of systematic bacteriology

Novel nitrogen-fixing acetic acid bacteria, Gluconacetobacter johannae sp. nov. and Gluconacetobacter azotocaptans sp. nov. associated with coffee plants= International Journal of Systematic and Evolutionary Microbiology (2001), 51, 1305–1314