Winery Lab Techniques

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Paper Chromatography for Monitoring Malolactic Fermentations

(Note: Chromatography reagent will stain your hands and clothes.  Use gloves and a lab coat to avoid stains.  Always wear splash goggles when working with chemicals)

1        Preparation of the chromatography solvent:

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Methylene blue was first identified in 1876 by Heinrich Caro, a German chemist. It has since been used in many applications, from an antidepressant, to a cure for cyanide poisoning, to a titration indicator in a laboratory.

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This method is to find whether a bacteria is gram negative (thin peptidoglycan layer in the cell wall) or positive (Thick peptidoglycan layer in the cell wall).

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